Kaylayla Raine was born in Vancouver and is a Canadian citizen. Raine has English, Scottish, and Squamish (Sko-ko-mish) roots.

Raine made her debut in 2017 in the short film Fight. In 2019, she played the role of Kaitlin in six episodes of the miniseries Hospital Show and took on a guest role in the television series Upload in 2020. Since 2021, she has appeared in the SyFy series Resident Alien as Jay, the daughter of Asta Twelvetrees.